Mechanome-guided Musculoskeletal (MSK)Tissue Engineering and Regenerative Rehabilitation
An important research trust of EBSL is the Mechanome-guided Musculoskeletal (MSK)Tissue Engineering and Regenerative Rehabilitation of tendon, annulus fibrosus, and intervertebral disc (IVD). Tendon injuries and intervertebral disc degeneration are common clinical problems that can lead to significant pain, disability, and decreased quality of life. Effective tissue regeneration strategies for these studies are only possible through understanding the mechanome (the mechanical forces and cues) involved in these tissues' normal function and healing processes. Towards this end, in mechanome-guided MSK tissue regeneration, we are guiding the differentiation of mesenchymal stem cells with various uniaxial and biaxial mechanical strains with different amplitudes and frequencies native to the targeted tissue environment for functional and stronger tissue repair. We are utilizing custom built and validated mechanical loading platform for in vitro studies and custom-built organ culture bioreactor for ex-vivo studies.
Selected List of Publications on MSK Tissue Engineering and Regenerative Rehabilitation
1) (2022) Jacho D, Rabino A, Garcia-Mata R, Yildirim-Ayan E. Mechanoresponsive regulation of fibroblast-to-myofibroblast transition in three-dimensional tissue analogues: mechanical strain amplitude dependefncy of fibrosis. Sci Rep. 2022 Oct 7;12(1):16832. PubMed Central ID: PMC9547073.
2) (2017) Subramanian G, Stasuk A, Elsaadany M, Yildirim-Ayan E. Effect of Uniaxial Tensile Cyclic Loading Regimes on Matrix Organization and Tenogenic Differentiation of Adipose-Derived Stem Cells Encapsulated within 3D Collagen Scaffolds. Stem Cells Int. 2017;2017:6072406. PubMed Central ID: PMC5742457.
3) (2017) Elsaadany M, Winters K, Adams S, Stasuk A, Ayan H, Yildirim-Ayan E. Equiaxial Strain Modulates Adipose-derived Stem Cell Differentiation within 3D Biphasic Scaffolds towards Annulus Fibrosus. Sci Rep. 2017 Oct 9;7(1):12868. PubMed Central ID: PMC5634474.
4) (2017) Subramanian G, Elsaadany M, Bialorucki C, Yildirim-Ayan E. Creating homogenous strain distribution within 3D cell-encapsulated constructs using a simple and cost-effective uniaxial tensile bioreactor: Design and validation study. Biotechnol Bioeng. 2017 Aug;114(8):1878-1887. PubMed ID: 28425561.
2) (2017) Subramanian G, Stasuk A, Elsaadany M, Yildirim-Ayan E. Effect of Uniaxial Tensile Cyclic Loading Regimes on Matrix Organization and Tenogenic Differentiation of Adipose-Derived Stem Cells Encapsulated within 3D Collagen Scaffolds. Stem Cells Int. 2017;2017:6072406. PubMed Central ID: PMC5742457.
3) (2017) Elsaadany M, Winters K, Adams S, Stasuk A, Ayan H, Yildirim-Ayan E. Equiaxial Strain Modulates Adipose-derived Stem Cell Differentiation within 3D Biphasic Scaffolds towards Annulus Fibrosus. Sci Rep. 2017 Oct 9;7(1):12868. PubMed Central ID: PMC5634474.
4) (2017) Subramanian G, Elsaadany M, Bialorucki C, Yildirim-Ayan E. Creating homogenous strain distribution within 3D cell-encapsulated constructs using a simple and cost-effective uniaxial tensile bioreactor: Design and validation study. Biotechnol Bioeng. 2017 Aug;114(8):1878-1887. PubMed ID: 28425561.
